› 3sqi

X-ray diffraction
2.82Å resolution

DNA binding domain of Ndc10

Released:
DOI: 10.2210/pdb3sqi/pdb
PDB entry 3sqi coloured by chain, front view.
PDB entry 3sqi coloured by chain, side view.
PDB entry 3sqi coloured by chain, top view.
Source organism: Kluyveromyces lactis NRRL Y-1140
Primary publication:
Ndc10 is a platform for inner kinetochore assembly in budding yeast.
Cho US, Harrison SC
Nat Struct Mol Biol 19 48-55 (2011)
PMID: 22139014

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116893 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
KLLA0E03807p Chain: A
Molecule details ›
Chain: A
Length: 534 amino acids
Theoretical weight: 61.51 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6CPM4 (Residues: 1-534; Coverage: 73%)
Gene name: KLLA0_E03807g
Sequence domains: Centromere DNA-binding protein complex CBF3 subunit, domain 2
Structure domains:
DNA (5'-D(P*TP*TP*AP*AP*TP*TP*TP*AP*TP*AP*AP*AP*AP*TP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided
DNA (5'-D(P*AP*AP*AP*TP*TP*TP*TP*AP*TP*AP*AP*AP*TP*TP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Kluyveromyces lactis NRRL Y-1140
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2221
Unit cell:
a: 117.303Å b: 147.536Å c: 95.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.193 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

A Molecular View of Kinetochore Assembly and Function.
Musacchio et al. (2017)
9 more

3 mentions without citation

Ndc10 is a platform for inner kinetochore assembly in budding yeast.
Cho et al. (2011)
2 more