› 3sqw

X-ray diffraction
1.91Å resolution

Structure of Mss116p (NTE deletion) bound to ssRNA and AMP-PNP

Released:
DOI: 10.2210/pdb3sqw/pdb
PDB entry 3sqw coloured by chain, front view.
PDB entry 3sqw coloured by chain, side view.
PDB entry 3sqw coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
High-throughput genetic identification of functionally important regions of the yeast DEAD-box protein Mss116p.
Mohr G, Del Campo M, Turner KG, Gilman B, Wolf RZ, Lambowitz AM
J Mol Biol 413 952-72 (2011)
PMID: 21945532

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147355 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
ATP-dependent RNA helicase MSS116, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 579 amino acids
Theoretical weight: 66.23 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15424 (Residues: 88-664; Coverage: 87%)
Gene names: MSS116, YD9346.05C, YDR194C
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ANP 1 x ANP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21212
Unit cell:
a: 88.159Å b: 126.63Å c: 56.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.237
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Toward a molecular understanding of RNA remodeling by DEAD-box proteins.
Russell et al. (2013)
2 more

1 mention without citation

High-throughput genetic identification of functionally important regions of the yeast DEAD-box protein Mss116p.
Mohr et al. (2011)