› 3sr6

X-ray diffraction
2.1Å resolution

Crystal Structure of Reduced Bovine Xanthine Oxidase in Complex with Arsenite

Released:
DOI: 10.2210/pdb3sr6/pdb
PDB entry 3sr6 coloured by chain, front view.
PDB entry 3sr6 coloured by chain, side view.
PDB entry 3sr6 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
X-ray crystal structure of arsenite-inhibited xanthine oxidase: μ-sulfido,μ-oxo double bridge between molybdenum and arsenic in the active site.
Cao H, Hall J, Hille R
J Am Chem Soc 133 12414-7 (2011)
PMID: 21761899

Function and Biology Details

Reactions catalysed: 
Xanthine + NAD(+) + H(2)O = urate + NADH
Xanthine + H(2)O + O(2) = urate + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160414 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Xanthine dehydrogenase/oxidase Chains: A, J
Molecule details ›
Chains: A, J
Length: 164 amino acids
Theoretical weight: 18 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 2-165; Coverage: 12%)
Gene name: XDH
Sequence domains:
Structure domains:
Xanthine dehydrogenase/oxidase Chains: B, K
Molecule details ›
Chains: B, K
Length: 305 amino acids
Theoretical weight: 34.02 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 224-528; Coverage: 23%)
Gene name: XDH
Sequence domains:
Structure domains:
Xanthine dehydrogenase/oxidase Chains: C, L
Molecule details ›
Chains: C, L
Length: 745 amino acids
Theoretical weight: 82.05 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80457 (Residues: 571-1315; Coverage: 56%)
Gene name: XDH
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand MTE 2 x MTE

Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand FES 4 x FES
Ligand RMO 2 x RMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 133.046Å b: 73.236Å c: 138.023Å
α: 90° β: 96.8° γ: 90°
R-values:
R R work R free
0.227 0.225 0.272

Quick links

Citations

3 review citations

The mononuclear molybdenum enzymes.
Hille et al. (2014)
2 more

2 mentions without citation

Structural data on the periplasmic aldehyde oxidoreductase PaoABC from Escherichia coli: SAXS and preliminary X-ray crystallography analysis.
Otrelo-Cardoso et al. (2014)
1 more