› 3srf

X-ray diffraction
2.84Å resolution

Human M1 pyruvate kinase

Released:
DOI: 10.2210/pdb3srf/pdb
PDB entry 3srf coloured by chain, front view.
PDB entry 3srf coloured by chain, side view.
PDB entry 3srf coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Morgan HP, O'Reilly F, Palmer R, McNae IW, Nowicki MW, Wear MA, Fothergill-Gilmore LA, Walkinshaw MD

Function and Biology Details

Reactions catalysed: 
ATP + pyruvate = ADP + phosphoenolpyruvate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146985 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pyruvate kinase PKM Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 551 amino acids
Theoretical weight: 60.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14618 (Residues: 1-531; Coverage: 100%)
Gene names: OIP3, PK2, PK3, PKM, PKM2
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand PO4 8 x PO4
Ligand K 8 x K
Ligand MG 8 x MG
Ligand PYR 8 x PYR
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 238.003Å b: 192.222Å c: 109.06Å
α: 90° β: 89.999° γ: 90°
R-values:
R R work R free
0.224 0.221 0.28
Expression system: Escherichia coli

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