› 3srp

X-ray diffraction
2.14Å resolution

Structure of Rivax: A Human Ricin Vaccine

Released:
DOI: 10.2210/pdb3srp/pdb
PDB entry 3srp coloured by chain, front view.
PDB entry 3srp coloured by chain, side view.
PDB entry 3srp coloured by chain, top view.
Source organism: Ricinus communis
Primary publication:
Structure of RiVax: a recombinant ricin vaccine.
Legler PM, Brey RN, Smallshaw JE, Vitetta ES, Millard CB
Acta Crystallogr D Biol Crystallogr 67 826-30 (2011)
PMID: 21904036

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ricin A chain Chain: A
Molecule details ›
Chain: A
Length: 268 amino acids
Theoretical weight: 30.01 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P02879 (Residues: 36-302; Coverage: 49%)
Sequence domains: Ribosome inactivating protein
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P41212
Unit cell:
a: 66.56Å b: 66.56Å c: 136.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.216 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein Structure Facilitates High-Resolution Immunological Mapping.
Zuverink et al. (2017)
10 other articles

12 mentions without citation

Progress and challenges associated with the development of ricin toxin subunit vaccines.
Vance et al. (2016)
11 more