› 3stb

X-ray diffraction
2.5Å resolution

A complex of two editosome proteins and two nanobodies

Released:
DOI: 10.2210/pdb3stb/pdb
PDB entry 3stb coloured by chain, front view.
PDB entry 3stb coloured by chain, side view.
PDB entry 3stb coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody.
Park YJ, Pardon E, Wu M, Steyaert J, Hol WG
Nucleic Acids Res 40 1828-40 (2012)
PMID: 22039098

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-212222 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
single domain antibody VHH Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 14.27 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
C2H2-type domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 148 amino acids
Theoretical weight: 16.89 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q95W13 (Residues: 247-393; Coverage: 37%)
Structure domains: Nucleic acid-binding proteins
MP18 RNA editing complex protein Chain: D
Molecule details ›
Chain: D
Length: 145 amino acids
Theoretical weight: 15.84 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q38B90 (Residues: 20-164; Coverage: 88%)
Gene name: Tb10.70.2090
Sequence domains: Single-strand binding protein family
Structure domains: Nucleic acid-binding proteins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2, null
Spacegroup: P43212
Unit cell:
a: 74.54Å b: 74.54Å c: 239.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.234
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Nanobodies as Probes for Protein Dynamics in Vitro and in Cells.
Dmitriev et al. (2016)
3 more

7 mentions without citation

Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody.
Park et al. (2012)
6 more