› 3stw

X-ray diffraction
2.31Å resolution

Crystal Structure of tomato Methylketone Synthase I complexed with 2-tridecanone

Released:
DOI: 10.2210/pdb3stw/pdb
PDB entry 3stw coloured by chain, front view.
PDB entry 3stw coloured by chain, side view.
PDB entry 3stw coloured by chain, top view.
Source organism: Solanum habrochaites
Primary publication:
Emergent decarboxylase activity and attenuation of α/β-hydrolase activity during the evolution of methylketone biosynthesis in tomato.
Auldridge ME, Guo Y, Austin MB, Ramsey J, Fridman E, Pichersky E, Noel JP
Plant Cell 24 1596-607 (2012)
PMID: 22523203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-122730 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 29.13 KDa
Source organism: Solanum habrochaites
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: E0YCS2 (Residues: 1-265; Coverage: 100%)
Gene name: MKS1
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand 2TD 2 x 2TD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 48.532Å b: 106.915Å c: 60.097Å
α: 90° β: 96.52° γ: 90°
R-values:
R R work R free
0.218 0.198 0.245
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Unveiling the functional diversity of the alpha/beta hydrolase superfamily in the plant kingdom.
Mindrebo et al. (2016)
1 more