› 3sux

X-ray diffraction
2.9Å resolution

Crystal structure of THF riboswitch, bound with THF

Released:
DOI: 10.2210/pdb3sux/pdb
PDB entry 3sux coloured by chain, front view.
PDB entry 3sux coloured by chain, side view.
PDB entry 3sux coloured by chain, top view.
Source organism: [Eubacterium] siraeum V10Sc8a
Primary publication:
Long-range pseudoknot interactions dictate the regulatory response in the tetrahydrofolate riboswitch.
Huang L, Ishibe-Murakami S, Patel DJ, Serganov A
Proc Natl Acad Sci U S A 108 14801-6 (2011)
PMID: 21873197

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-196742 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
Riboswitch Chain: X
Molecule details ›
Chain: X
Length: 101 nucleotides
Theoretical weight: 32.84 KDa
Source organism: [Eubacterium] siraeum V10Sc8a
Expression system: Not provided
Sequence domains: THF riboswitch

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand THF 1 x THF
1 modified residue:
Ligand CCC 1 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 126.173Å b: 126.173Å c: 149.222Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.232
Expression system: Not provided

Quick links

Citations

9 review citations

A decade of riboswitches.
Serganov et al. (2013)
8 more

11 mentions without citation

Structure and function of pseudoknots involved in gene expression control.
Peselis et al. (2014)
10 more