› 3svl

X-ray diffraction
2.2Å resolution

Structural basis of the improvement of ChrR - a multi-purpose enzyme

Released:
DOI: 10.2210/pdb3svl/pdb
PDB entry 3svl coloured by chain, front view.
PDB entry 3svl coloured by chain, side view.
PDB entry 3svl coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Crystal structure of ChrR--a quinone reductase with the capacity to reduce chromate.
Eswaramoorthy S, Poulain S, Hienerwadel R, Bremond N, Sylvester MD, Zhang YB, Berthomieu C, Van Der Lelie D, Matin A
PLoS One 7 e36017 (2012)
PMID: 22558308

Function and Biology Details

Reaction catalysed: 
NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142828 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Quinone reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 193 amino acids
Theoretical weight: 20.85 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGE6 (Residues: 1-188; Coverage: 100%)
Gene names: JW3691, b3713, chrR, yieF
Sequence domains: NADPH-dependent FMN reductase
Structure domains: Rossmann fold

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6322
Unit cell:
a: 107.255Å b: 107.255Å c: 128.251Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.257 0.237 0.27
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium: a review.
Thatoi et al. (2014)
3 more

7 mentions without citation

Crystal structure of ChrR--a quinone reductase with the capacity to reduce chromate.
Eswaramoorthy et al. (2012)
6 more