› 3svn

X-ray diffraction
1.9Å resolution

Crystal structure of mKate S158A mutant at pH 7.5

Released:
DOI: 10.2210/pdb3svn/pdb
PDB entry 3svn coloured by chain, front view.
PDB entry 3svn coloured by chain, side view.
PDB entry 3svn coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Molecular mechanism of a green-shifted, pH-dependent red fluorescent protein mKate variant.
Wang Q, Byrnes LJ, Shui B, Röhrig UF, Singh A, Chudakov DM, Lukyanov S, Zipfel WR, Kotlikoff MI, Sondermann H
PLoS One 6 e23513 (2011)
PMID: 21887263

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-164045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mKate Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 26.25 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 4 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: I41
Unit cell:
a: 161.328Å b: 161.328Å c: 76.183Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.172 0.221
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
6 other articles

1 mention without citation

Molecular mechanism of a green-shifted, pH-dependent red fluorescent protein mKate variant.
Wang et al. (2011)