› 3svr

X-ray diffraction
1.91Å resolution

Crystal structure of mkate mutant S158A/S143C at pH 7.5

Released:
DOI: 10.2210/pdb3svr/pdb
PDB entry 3svr coloured by chain, front view.
PDB entry 3svr coloured by chain, side view.
PDB entry 3svr coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Molecular mechanism of a green-shifted, pH-dependent red fluorescent protein mKate variant.
Wang Q, Byrnes LJ, Shui B, Röhrig UF, Singh A, Chudakov DM, Lukyanov S, Zipfel WR, Kotlikoff MI, Sondermann H
PLoS One 6 e23513 (2011)
PMID: 21887263

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-164049 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mkate S158A/S143C Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 233 amino acids
Theoretical weight: 26.26 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 4 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: I41
Unit cell:
a: 161.31Å b: 161.31Å c: 75.441Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.227
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Directed molecular evolution to design advanced red fluorescent proteins.
Subach et al. (2011)
5 more

2 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
1 more