› 3svs

X-ray diffraction
1.74Å resolution

Crystal structure of mkate mutant S158A/S143C at pH 4.0

Released:
DOI: 10.2210/pdb3svs/pdb
PDB entry 3svs coloured by chain, front view.
PDB entry 3svs coloured by chain, side view.
PDB entry 3svs coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Molecular mechanism of a green-shifted, pH-dependent red fluorescent protein mKate variant.
Wang Q, Byrnes LJ, Shui B, Röhrig UF, Singh A, Chudakov DM, Lukyanov S, Zipfel WR, Kotlikoff MI, Sondermann H
PLoS One 6 e23513 (2011)
PMID: 21887263

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-164051 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mKate S158A/S143C Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 233 amino acids
Theoretical weight: 26.26 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 8 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 68.708Å b: 101.966Å c: 276.474Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.199 0.236
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Directed molecular evolution to design advanced red fluorescent proteins.
Subach et al. (2011)
5 more

2 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
1 more