› 3swn

X-ray diffraction
2.5Å resolution

Structure of the LSm657 Complex: An Assembly Intermediate of the LSm1 7 and LSm2 8 Rings

Released:
DOI: 10.2210/pdb3swn/pdb
PDB entry 3swn coloured by chain, front view.
PDB entry 3swn coloured by chain, side view.
PDB entry 3swn coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Structure of the LSm657 complex: an assembly intermediate of the LSm1-7 and LSm2-8 rings.
Mund M, Neu A, Ullmann J, Neu U, Sprangers R
J Mol Biol 414 165-76 (2011)
PMID: 22001694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
PDBe Complex ID:
PDB-CPX-128820 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
U6 snRNA-associated Sm-like protein LSm7 Chains: C, F, O, R
Molecule details ›
Chains: C, F, O, R
Length: 117 amino acids
Theoretical weight: 12.8 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O74499 (Residues: 1-113; Coverage: 100%)
Gene names: SPCC285.12, lsm7
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
U6 snRNA-associated Sm-like protein LSm5 Chains: A, D, P, S
Molecule details ›
Chains: A, D, P, S
Length: 82 amino acids
Theoretical weight: 9.05 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: O42978 (Residues: 1-80; Coverage: 100%)
Gene names: SPBC20F10.09, lsm5
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
U6 snRNA-associated Sm-like protein LSm6 Chains: B, E, Q, T
Molecule details ›
Chains: B, E, Q, T
Length: 77 amino acids
Theoretical weight: 8.53 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UUI1 (Residues: 1-75; Coverage: 100%)
Gene names: SPAC2F3.17c, lsm6
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

1 bound ligand:
Ligand ZN 16 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 79.53Å b: 63.1Å c: 92.76Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.221 0.218 0.269
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Structural and functional control of the eukaryotic mRNA decapping machinery.
Arribas-Layton et al. (2013)
3 more