› 3sya

X-ray diffraction
2.98Å resolution

Crystal structure of the G protein-gated inward rectifier K+ channel GIRK2 (Kir3.2) in complex with sodium and PIP2

Released:
DOI: 10.2210/pdb3sya/pdb
PDB entry 3sya coloured by chain, front view.
PDB entry 3sya coloured by chain, side view.
PDB entry 3sya coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the mammalian GIRK2 K+ channel and gating regulation by G proteins, PIP2, and sodium.
Whorton MR, MacKinnon R
Cell 147 199-208 (2011)
PMID: 21962516

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
G protein-activated inward rectifier potassium channel 2 Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 39.06 KDa
Source organism: Mus musculus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P48542 (Residues: 52-380; Coverage: 77%)
Gene names: Girk2, Kcnj6, Kcnj7, W
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand PIO 1 x PIO
Ligand K 5 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4212
Unit cell:
a: 85.851Å b: 85.851Å c: 177.862Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.269
Expression system: Komagataella pastoris

Quick links

Citations

61 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
60 more

48 mentions without citation

Structure of potassium channels.
Kuang et al. (2015)
47 more