› 3sys

X-ray diffraction
1.65Å resolution

Improved crystal structure of Pseudomonas aeruginosa OccK1 (OpdK)

Released:
DOI: 10.2210/pdb3sys/pdb
PDB entry 3sys coloured by chain, front view.
PDB entry 3sys coloured by chain, side view.
PDB entry 3sys coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Substrate specificity within a family of outer membrane carboxylate channels.
Eren E, Vijayaraghavan J, Liu J, Cheneke BR, Touw DS, Lepore BW, Indic M, Movileanu L, van den Berg B
PLoS Biol 10 e1001242 (2012)
PMID: 22272184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vanillate porin OpdK Chains: A, B
Molecule details ›
Chains: A, B
Length: 398 amino acids
Theoretical weight: 43.94 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HUR5 (Residues: 28-417; Coverage: 100%)
Gene names: PA4898, opdK
Sequence domains: outer membrane porin, OprD family
Structure domains: Porin

Ligands and Environments

3 bound ligands:
Ligand EDO 4 x EDO
Ligand C8E 29 x C8E
Ligand VNL 4 x VNL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 82.142Å b: 96.84Å c: 121.557Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.201
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
11 more

4 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
3 more