› 3szd

X-ray diffraction
2.31Å resolution

Crystal structure of Pseudomonas aeruginosa OccK2 (OpdF)

Released:
DOI: 10.2210/pdb3szd/pdb
PDB entry 3szd coloured by chain, front view.
PDB entry 3szd coloured by chain, side view.
PDB entry 3szd coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Substrate specificity within a family of outer membrane carboxylate channels.
Eren E, Vijayaraghavan J, Liu J, Cheneke BR, Touw DS, Lepore BW, Indic M, Movileanu L, van den Berg B
PLoS Biol 10 e1001242 (2012)
PMID: 22272184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191467 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable porin Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 44.71 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I6P8 (Residues: 25-421; Coverage: 100%)
Gene name: PA0240
Sequence domains: outer membrane porin, OprD family
Structure domains: Porin

Ligands and Environments

3 bound ligands:
Ligand C8E 5 x C8E
Ligand SO4 7 x SO4
Ligand 3PE 1 x 3PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P21
Unit cell:
a: 46.321Å b: 206.899Å c: 51.581Å
α: 90° β: 99.09° γ: 90°
R-values:
R R work R free
0.229 0.225 0.28
Expression system: Escherichia coli

Quick links

Citations

12 review citations

The challenge of efflux-mediated antibiotic resistance in Gram-negative bacteria.
Li et al. (2015)
11 more

6 mentions without citation

Multiscale Simulations of Biological Membranes: The Challenge To Understand Biological Phenomena in a Living Substance.
Enkavi et al. (2019)
5 more