› 3szp

X-ray diffraction
2.2Å resolution

Full-length structure of the Vibrio cholerae virulence activator, AphB, a member of the LTTR protein family

Released:
DOI: 10.2210/pdb3szp/pdb
PDB entry 3szp coloured by chain, front view.
PDB entry 3szp coloured by chain, side view.
PDB entry 3szp coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
The crystal structure of AphB, a virulence gene activator from Vibrio cholerae, reveals residues that influence its response to oxygen and pH.
Taylor JL, De Silva RS, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ
Mol Microbiol 83 457-70 (2012)
PMID: 22053934

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lysR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 33.36 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KT56 (Residues: 9-299; Coverage: 97%)
Gene name: VC_1049
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: I222
Unit cell:
a: 100.209Å b: 106.1Å c: 167.846Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.234
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Efficient responses to host and bacterial signals during Vibrio cholerae colonization.
Rothenbacher et al. (2014)
1 more

8 mentions without citation

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
7 more