› 3t1b

X-ray diffraction
2.7Å resolution

Crystal structure of the full-length AphB N100E variant

Released:
DOI: 10.2210/pdb3t1b/pdb
PDB entry 3t1b coloured by chain, front view.
PDB entry 3t1b coloured by chain, side view.
PDB entry 3t1b coloured by chain, top view.
Source organism: Vibrio cholerae
Primary publication:
The crystal structure of AphB, a virulence gene activator from Vibrio cholerae, reveals residues that influence its response to oxygen and pH.
Taylor JL, De Silva RS, Kovacikova G, Lin W, Taylor RK, Skorupski K, Kull FJ
Mol Microbiol 83 457-70 (2012)
PMID: 22053934

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH lysR-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 291 amino acids
Theoretical weight: 33.38 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KT56 (Residues: 9-299; Coverage: 97%)
Gene name: VC_1049
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 268.737Å b: 54.545Å c: 103.84Å
α: 90° β: 100.95° γ: 90°
R-values:
R R work R free
0.199 0.197 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Cell-Wall Recycling of the Gram-Negative Bacteria and the Nexus to Antibiotic Resistance.
Dik et al. (2018)
2 more

3 mentions without citation

The crystal structure of AphB, a virulence gene activator from Vibrio cholerae, reveals residues that influence its response to oxygen and pH.
Taylor et al. (2012)
2 more