› 3t2a

X-ray diffraction
2.1Å resolution

TMAO-grown cubic insulin (porcine)

Released:
DOI: 10.2210/pdb3t2a/pdb
PDB entry 3t2a coloured by chain, front view.
PDB entry 3t2a coloured by chain, side view.
PDB entry 3t2a coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.
Marshall H, Venkat M, Seng NS, Cahn J, Juers DH
Acta Crystallogr D Biol Crystallogr 68 69-81 (2012)
PMID: 22194335

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-134237 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand TMO 1 x TMO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: I213
Unit cell:
a: 79.098Å b: 79.098Å c: 79.098Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.223

Quick links

Citations

1 review citation

The impact of structural biology on alkaloid biosynthesis research.
Panjikar et al. (2012)
7 other articles

3 mentions without citation

The use of trimethylamine N-oxide as a primary precipitating agent and related methylamine osmolytes as cryoprotective agents for macromolecular crystallography.
Marshall et al. (2012)
2 more