› 3t32

X-ray diffraction
2Å resolution

Crystal structure of a putative C-S lyase from Bacillus anthracis

Released:
DOI: 10.2210/pdb3t32/pdb
PDB entry 3t32 coloured by chain, front view.
PDB entry 3t32 coloured by chain, side view.
PDB entry 3t32 coloured by chain, top view.
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Entry authors: Anderson SM, Wawrzak Z, Gordon E, Peterson SN, Porebski P, Minor W, Savchenko A, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(1a) an L-cysteine-S-conjugate = a thiol + 2-aminoporp-2-enoate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cysteine-S-conjugate beta-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 383 amino acids
Theoretical weight: 44.6 KDa
Source organism: Bacillus anthracis str. 'Ames Ancestor'
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A6L7H8L3 (Residues: 1-383; Coverage: 100%)
Gene name: GBAA_5138
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 14 x MSE
Ligand LLP 2 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 52.291Å b: 84.655Å c: 170.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.207
Expression system: Escherichia coli

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