› 3t40

X-ray diffraction
1.75Å resolution

Crystal Structure of Mycobacterium tuberculosis Indole Glycerol Phosphate Synthase (IGPS) complex with N-2-Carboxyphenyl Glycine (CPG)

Released:
DOI: 10.2210/pdb3t40/pdb
PDB entry 3t40 coloured by chain, front view.
PDB entry 3t40 coloured by chain, side view.
PDB entry 3t40 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Entry authors: Reddy MCM, Bruning JB, Thurman C, Sacchettini JC, TB Structural Genomics Consortium (TBSGC)

Function and Biology Details

Reaction catalysed: 
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO(2) + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161440 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Indole-3-glycerol phosphate synthase Chain: A
Molecule details ›
Chain: A
Length: 272 amino acids
Theoretical weight: 28.05 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WFX7 (Residues: 1-272; Coverage: 100%)
Gene names: MTCY01B2.03, Rv1611, trpC
Sequence domains: Indole-3-glycerol phosphate synthase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand 3RG 1 x 3RG
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 45.844Å b: 53.019Å c: 88.473Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.178 0.22
Expression system: Escherichia coli BL21(DE3)

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