› 3t4k

X-ray diffraction
1.77Å resolution

Arabidopsis histidine kinase 4 sensor domain in complex with N-benzyladenine

Released:
DOI: 10.2210/pdb3t4k/pdb
PDB entry 3t4k coloured by chain, front view.
PDB entry 3t4k coloured by chain, side view.
PDB entry 3t4k coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural basis for cytokinin recognition by Arabidopsis thaliana histidine kinase 4.
Hothorn M, Dabi T, Chory J
Nat Chem Biol 7 766-8 (2011)
PMID: 21964459

Function and Biology Details

Reactions catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189921 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histidine kinase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 270 amino acids
Theoretical weight: 30.74 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9C5U0 (Residues: 149-418; Coverage: 25%)
Gene names: AHK4, At2g01830, CRE1, RAW1, T23K3.2, WOL
Sequence domains: CHASE domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EMU 2 x EMU
Ligand MLI 1 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3221
Unit cell:
a: 60.14Å b: 60.14Å c: 297.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.21
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Cytokinin signaling in plant development.
Kieber et al. (2018)
21 more

5 mentions without citation

Structural basis for cytokinin recognition by Arabidopsis thaliana histidine kinase 4.
Hothorn et al. (2011)
4 more