› 3t56

X-ray diffraction
3.42Å resolution

Crystal structure of the pre-extrusion state of the CusBA adaptor-transporter complex

Released:
DOI: 10.2210/pdb3t56/pdb
PDB entry 3t56 coloured by chain, front view.
PDB entry 3t56 coloured by chain, side view.
PDB entry 3t56 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Charged amino acids (R83, E567, D617, E625, R669, and K678) of CusA are required for metal ion transport in the Cus efflux system.
Su CC, Long F, Lei HT, Bolla JR, Do SV, Rajashankar KR, Yu EW
J Mol Biol 422 429-41 (2012)
PMID: 22683351

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero nonamer
Assembly name:
PDBe Complex ID:
PDB-CPX-153535 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cation efflux system protein CusA Chain: A
Molecule details ›
Chain: A
Length: 1054 amino acids
Theoretical weight: 115.7 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38054 (Residues: 1-1047; Coverage: 100%)
Gene names: JW0564, b0575, cusA, ybdE
Sequence domains: AcrB/AcrD/AcrF family
Cation efflux system protein CusB Chains: B, C
Molecule details ›
Chains: B, C
Length: 336 amino acids
Theoretical weight: 36.62 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77239 (Residues: 78-407; Coverage: 87%)
Gene names: JW0563, b0574, cusB, ylcD
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CU 1 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: R32
Unit cell:
a: 160.121Å b: 160.121Å c: 684.945Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.261 0.259 0.294
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli

Quick links

Citations

4 review citations

Similarities and Differences between Silver Ions and Silver in Nanoforms as Antibacterial Agents.
Kędziora et al. (2018)
3 more

6 mentions without citation

Bacterial multidrug efflux transporters.
Delmar et al. (2014)
5 more