› 3t5n

X-ray diffraction
1.79Å resolution

1.8A crystal structure of Lassa virus nucleoprotein in complex with ssRNA

Released:
DOI: 10.2210/pdb3t5n/pdb
PDB entry 3t5n coloured by chain, front view.
PDB entry 3t5n coloured by chain, side view.
PDB entry 3t5n coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of the Lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding.
Hastie KM, Liu T, Li S, King LB, Ngo N, Zandonatti MA, Woods VL, de la Torre JC, Saphire EO
Proc Natl Acad Sci U S A 108 19365-70 (2011)
PMID: 22084115

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177803 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 354 amino acids
Theoretical weight: 39.28 KDa
Source organism: Mopeia Lassa virus reassortant 29
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S585 (Residues: 1-340; Coverage: 60%)
Gene names: KUE_IGS110004, KUE_IGS130004, KUE_IGS160004, N, NP
Sequence domains: Arenavirus nucleocapsid N-terminal domain
RNA (5'-R(P*UP*AP*UP*CP*UP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 6 nucleotides
Theoretical weight: 1.81 KDa
Source organism: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21212
Unit cell:
a: 74.159Å b: 71.737Å c: 56.887Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.21
Expression system: Escherichia coli

Quick links

Citations

27 review citations

The SARS coronavirus nucleocapsid protein--forms and functions.
Chang et al. (2014)
26 more

6 mentions without citation

Crystal structure of the Lassa virus nucleoprotein-RNA complex reveals a gating mechanism for RNA binding.
Hastie et al. (2011)
5 more