› 3t65

X-ray diffraction
1.45Å resolution

S25-2- A(2-8)KDO disaccharide complex

Released:
DOI: 10.2210/pdb3t65/pdb
PDB entry 3t65 coloured by chain, front view.
PDB entry 3t65 coloured by chain, side view.
PDB entry 3t65 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Germline antibody recognition of distinct carbohydrate epitopes.
Nguyen HP, Seto NO, MacKenzie CR, Brade L, Kosma P, Brade H, Evans SV
Nat Struct Biol 10 1019-25 (2003)
PMID: 14625588

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213403 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
S25-2 FAB (IGG1K) heavy chain Chain: B
Molecule details ›
Chain: B
Length: 222 amino acids
Theoretical weight: 24.11 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
S25-2 FAB (IGG1K) light chain Chain: A
Molecule details ›
Chain: A
Length: 219 amino acids
Theoretical weight: 24.24 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: KDA, KDO
Ligand KDA 1 x KDA
Ligand KDO 1 x KDO
2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 45.9Å b: 81.6Å c: 131.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.193 0.211
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Glycan array: a powerful tool for glycomics studies.
Liang et al. (2009)
4 more

3 mentions without citation

Blind prediction performance of RosettaAntibody 3.0: grafting, relaxation, kinematic loop modeling, and full CDR optimization.
Weitzner et al. (2014)
2 more