› 3t72

X-ray diffraction
4.33Å resolution

PhoB(E)-Sigma70(4)-(RNAP-Betha-flap-tip-helix)-DNA Transcription Activation Sub-Complex

Released:
DOI: 10.2210/pdb3t72/pdb
PDB entry 3t72 coloured by chain, front view.
PDB entry 3t72 coloured by chain, side view.
PDB entry 3t72 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The structure of a transcription activation subcomplex reveals how σ(70) is recruited to PhoB promoters.
Blanco AG, Canals A, Bernués J, Solà M, Coll M
EMBO J 30 3776-85 (2011)
PMID: 21829166

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-116915 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Phosphate regulon transcriptional regulatory protein PhoB Chains: 1, 4, 5, 8, 9, A, B, E, F, I, J, M, N, R, S, V, W, Z, c, d, g, h, k, l
Molecule details ›
Chains: 1, 4, 5, 8, 9, A, B, E, F, I, J, M, N, R, S, V, W, Z, c, d, g, h, k, l
Length: 102 amino acids
Theoretical weight: 11.97 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AFJ5 (Residues: 128-229; Coverage: 45%)
Gene names: JW0389, b0399, phoB
Sequence domains: Transcriptional regulatory protein, C terminal
DNA-directed RNA polymerase subunit beta; RNA polymerase sigma factor RpoD Chains: o, q
Molecule details ›
Chains: o, q
Length: 99 amino acids
Theoretical weight: 11.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00579 (Residues: 533-609; Coverage: 13%)
  • Canonical: P0A8V2 (Residues: 896-910; Coverage: 1%)
Gene names: JW3039, JW3950, alt, b3067, b3987, groN, nitB, rif, ron, rpoB, rpoD, stl, stv, tabD
Sequence domains: Sigma-70, region 4
PHO BOX DNA (STRAND 1) Chains: 2, 6, C, G, K, O, T, X, a, e, i, m
Molecule details ›
Chains: 2, 6, C, G, K, O, T, X, a, e, i, m
Length: 26 nucleotides
Theoretical weight: 8.04 KDa
PHO BOX DNA (STRAND 2) Chains: 3, 7, D, H, L, P, U, Y, b, f, j, n
Molecule details ›
Chains: 3, 7, D, H, L, P, U, Y, b, f, j, n
Length: 26 nucleotides
Theoretical weight: 7.94 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 277.3Å b: 161.4Å c: 260.1Å
α: 90° β: 91.4° γ: 90°
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The Pho regulon: a huge regulatory network in bacteria.
Santos-Beneit F. (2015)
3 more

9 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
8 more