› 3t8l

X-ray diffraction
2.8Å resolution

Crystal Structure of adenine deaminase with Mn/Fe

Released:
DOI: 10.2210/pdb3t8l/pdb
PDB entry 3t8l coloured by chain, front view.
PDB entry 3t8l coloured by chain, side view.
PDB entry 3t8l coloured by chain, top view.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
The catalase activity of diiron adenine deaminase.
Kamat SS, Holmes-Hampton GP, Bagaria A, Kumaran D, Tichy SE, Gheyi T, Zheng X, Bain K, Groshong C, Emtage S, Sauder JM, Burley SK, Swaminathan S, Lindahl PA, Raushel FM
Protein Sci 20 2080-94 (2011)
PMID: 21998098

Function and Biology Details

Reaction catalysed: 
Adenine + H(2)O = hypoxanthine + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181443 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenine deaminase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 608 amino acids
Theoretical weight: 65.07 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7CUX4 (Residues: 1-597; Coverage: 100%)
Gene names: AGR_L_883, Atu4426, ade2, adeC
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UNX 6 x UNX
1 modified residue:
Ligand MSE 36 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 61.543Å b: 131.171Å c: 69.284Å
α: 90° β: 97.04° γ: 90°
R-values:
R R work R free
0.181 0.174 0.301
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Transcriptome analysis of Enterococcus faecalis in response to alkaline stress.
Ran et al. (2015)
2 more

1 mention without citation

The catalase activity of diiron adenine deaminase.
Kamat et al. (2011)