› 3t8o

X-ray diffraction
2.5Å resolution

Rhodopsin kinase (GRK1) L166K mutant at 2.5A resolution

Released:
DOI: 10.2210/pdb3t8o/pdb
PDB entry 3t8o coloured by chain, front view.
PDB entry 3t8o coloured by chain, side view.
PDB entry 3t8o coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of a monomeric variant of rhodopsin kinase at 2.5 Å resolution.
Tesmer JJ, Nance MR, Singh P, Lee H
Acta Crystallogr Sect F Struct Biol Cryst Commun 68 622-5 (2012)
PMID: 22684056

Function and Biology Details

Reaction catalysed: 
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151205 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rhodopsin kinase GRK1 Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 61.45 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28327 (Residues: 1-535; Coverage: 95%)
Gene names: GRK1, RHOK
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 1 x MG
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: I222
Unit cell:
a: 55.541Å b: 149.808Å c: 190.879Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.245
Expression system: Spodoptera frugiperda

Quick links

Citations

3 citation in other articles

Crystal Structure of G Protein-coupled Receptor Kinase 5 in Complex with a Rationally Designed Inhibitor.
Homan et al. (2015)
2 more

2 mentions without citation

Structural view of G protein-coupled receptor signaling in the retinal rod outer segment.
Gulati et al. (2023)
1 more