› 3t9n

X-ray diffraction
3.46Å resolution

Crystal structure of a membrane protein

Released:
DOI: 10.2210/pdb3t9n/pdb
PDB entry 3t9n coloured by chain, front view.
PDB entry 3t9n coloured by chain, side view.
PDB entry 3t9n coloured by chain, top view.
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Primary publication:
Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance.
Zhang X, Wang J, Feng Y, Ge J, Li W, Sun W, Iscla I, Yu J, Blount P, Li Y, Yang M
Proc Natl Acad Sci U S A 109 18180-5 (2012)
PMID: 23074248

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185927 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Small-conductance mechanosensitive channel Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 282 amino acids
Theoretical weight: 32.07 KDa
Source organism: Caldanaerobacter subterraneus subsp. tengcongensis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8R6L9 (Residues: 1-282; Coverage: 100%)
Gene names: TTE2783, mscS
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LMT 2 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 95.029Å b: 138.627Å c: 214.58Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.254 0.253 0.288
Expression system: Escherichia coli

Quick links

Citations

12 review citations

United in diversity: mechanosensitive ion channels in plants.
Hamilton et al. (2015)
11 more

9 mentions without citation

Open and shut: crystal structures of the dodecylmaltoside solubilized mechanosensitive channel of small conductance from Escherichia coli and Helicobacter pylori at 4.4 Å and 4.1 Å resolutions.
Lai et al. (2013)
8 more