› 3tb7

X-ray diffraction
2.45Å resolution

The type I crystal structure of Streptococcus agalactiae sortase C1

Released:
DOI: 10.2210/pdb3tb7/pdb
PDB entry 3tb7 coloured by chain, front view.
PDB entry 3tb7 coloured by chain, side view.
PDB entry 3tb7 coloured by chain, top view.
Source organism: Streptococcus agalactiae serogroup V
Primary publication:
The crystal structure analysis of group B Streptococcus sortase C1: a model for the "lid" movement upon substrate binding.
Khare B, Fu ZQ, Huang IH, Ton-That H, Narayana SV
J Mol Biol 414 563-77 (2011)
PMID: 22033482

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sortase family protein Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 25.78 KDa
Source organism: Streptococcus agalactiae serogroup V
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8E0S7 (Residues: 43-260; Coverage: 72%)
Gene name: SAG0647
Sequence domains: Sortase domain
Structure domains: Sortase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: C2
Unit cell:
a: 78.59Å b: 46.93Å c: 66.57Å
α: 90° β: 114.99° γ: 90°
R-values:
R R work R free
0.232 0.228 0.308
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Molecular features of the sortase enzyme family.
Bradshaw et al. (2015)
1 more

3 mentions without citation

Sortase Transpeptidases: Structural Biology and Catalytic Mechanism.
Jacobitz et al. (2017)
2 more