› 3tbi

X-ray diffraction
3Å resolution

Crystal structure of T4 gp33 bound to E. coli RNAP beta-flap domain

Released:
DOI: 10.2210/pdb3tbi/pdb
PDB entry 3tbi coloured by chain, front view.
PDB entry 3tbi coloured by chain, side view.
PDB entry 3tbi coloured by chain, top view.
Source organisms:
Entry authors: Twist KAF, Campbell EA, Darst SA

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141749 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Late transcription coactivator Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 13.28 KDa
Source organism: Tequatrovirus T4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13338 (Residues: 1-112; Coverage: 100%)
Gene name: 33
Sequence domains: Phage late-transcription coactivator
Structure domains: Phage late-transcription coactivator-like
DNA-directed RNA polymerase subunit beta Chain: B
Molecule details ›
Chain: B
Length: 228 amino acids
Theoretical weight: 25.37 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8V2 (Residues: 831-1057; Coverage: 17%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains: RNA polymerase Rpb2, domain 6
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X3A
Spacegroup: I222
Unit cell:
a: 55.218Å b: 112.25Å c: 164.847Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.251 0.295
Expression system: Escherichia coli BL21(DE3)

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