› 3tbs

X-ray diffraction
2.49Å resolution

CRYSTAL STRUCTURE OF THE MURINE CLASS I MAJOR HISTOCOMPATIBILITY COMPLEX H-2DB IN COMPLEX THE WITH LCMV-DERIVED GP33 ALTERED PEPTIDE ligand (V3P,Y4A)

Released:
DOI: 10.2210/pdb3tbs/pdb
PDB entry 3tbs coloured by chain, front view.
PDB entry 3tbs coloured by chain, side view.
PDB entry 3tbs coloured by chain, top view.
Source organisms:
Entry authors: Duru AD, Allerbring EB, Uchtenhagen H, Mazumdar PA, Badia-Martinez D, Madhurantakam C, Sandalova T, Nygren P, Achour A

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-134985 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, D-B alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 276 amino acids
Theoretical weight: 32.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01899 (Residues: 25-300; Coverage: 82%)
Gene name: H2-D1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Stable signal peptide Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 951 Da
Source organism: Lymphocytic choriomeningitis virus (strain WE)
Expression system: Not provided
UniProt:
  • Canonical: P07399 (Residues: 33-41; Coverage: 2%)
Gene names: GP-C, GPC

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 117.84Å b: 125.12Å c: 101.32Å
α: 90° β: 128.15° γ: 90°
R-values:
R R work R free
0.232 0.23 0.27
Expression systems:
  • Escherichia coli
  • Not provided

Quick links