› 3tdm

X-ray diffraction
2.4Å resolution

Computationally designed TIM-barrel protein, HalfFLR

Released:
DOI: 10.2210/pdb3tdm/pdb
PDB entry 3tdm coloured by chain, front view.
PDB entry 3tdm coloured by chain, side view.
PDB entry 3tdm coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Exploring symmetry as an avenue to the computational design of large protein domains.
Fortenberry C, Bowman EA, Proffitt W, Dorr B, Combs S, Harp J, Mizoue L, Meiler J
J Am Chem Soc 133 18026-9 (2011)
PMID: 21978247

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Computationally designed two-fold symmetric TIM-barrel protein, FLR (half molecule) Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 126 amino acids
Theoretical weight: 13.58 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P43
Unit cell:
a: 60.493Å b: 60.493Å c: 140.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.242 0.288
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Protein Assembly by Design.
Zhu et al. (2021)
3 more

1 mention without citation

Energy functions in de novo protein design: current challenges and future prospects.
Li et al. (2013)