› 3tdn

X-ray diffraction
1.4Å resolution

Computationally designed two-fold symmetric Tim-barrel protein, FLR

Released:
DOI: 10.2210/pdb3tdn/pdb
PDB entry 3tdn coloured by chain, front view.
PDB entry 3tdn coloured by chain, side view.
PDB entry 3tdn coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Exploring symmetry as an avenue to the computational design of large protein domains.
Fortenberry C, Bowman EA, Proffitt W, Dorr B, Combs S, Harp J, Mizoue L, Meiler J
J Am Chem Soc 133 18026-9 (2011)
PMID: 21978247

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-164073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FLR SYMMETRIC ALPHA-BETA TIM BARREL Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 26.7 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 12.3.1
Spacegroup: P3221
Unit cell:
a: 67.222Å b: 67.222Å c: 124.658Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.157 0.193
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Energy functions in de novo protein design: current challenges and future prospects.
Li et al. (2013)
4 more

3 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
2 more