› 3tej

X-ray diffraction
1.9Å resolution

Crystal structure of a domain fragment involved in peptide natural product biosynthesis

Released:
DOI: 10.2210/pdb3tej/pdb
Model geometry
Fit model/data
PDB entry 3tej coloured by chain, front view.
PDB entry 3tej coloured by chain, side view.
PDB entry 3tej coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for phosphopantetheinyl carrier domain interactions in the terminal module of nonribosomal peptide synthetases.
Liu Y, Zheng T, Bruner SD
Chem Biol 18 1482-8 (2011)
PMID: 22118682

Function and Biology Details

Reactions catalysed: 
(1a) ATP + L-serine = diphosphate + (L-seryl)adenylate
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145843 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Enterobactin synthase component F Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.13 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11454 (Residues: 965-1293; Coverage: 25%)
Gene names: JW0578, b0586, entF
Sequence domains:
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
1 modified residue:
Ligand UF0 2 x UF0

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 69.4Å b: 90.36Å c: 97.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.206 0.227
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

Nonribosomal Peptide Synthesis-Principles and Prospects.
Süssmuth et al. (2017)
12 more

16 mentions without citation

Structure and noncanonical chemistry of nonribosomal peptide biosynthetic machinery.
Condurso et al. (2012)
15 more