› 3tg0

X-ray diffraction
1.2Å resolution

E. coli alkaline phosphatase with bound inorganic phosphate

Released:
DOI: 10.2210/pdb3tg0/pdb
PDB entry 3tg0 coloured by chain, front view.
PDB entry 3tg0 coloured by chain, side view.
PDB entry 3tg0 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
High-resolution analysis of Zn(2+) coordination in the alkaline phosphatase superfamily by EXAFS and x-ray crystallography.
Bobyr E, Lassila JK, Wiersma-Koch HI, Fenn TD, Lee JJ, Nikolic-Hughes I, Hodgson KO, Rees DC, Hedman B, Herschlag D
J Mol Biol 415 102-17 (2012)
PMID: 22056344

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkaline phosphatase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 449 amino acids
Theoretical weight: 47.09 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00634 (Residues: 23-471; Coverage: 100%)
Gene names: JW0374, b0383, phoA
Sequence domains: Alkaline phosphatase
Structure domains: Alkaline Phosphatase, subunit A

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand MG 4 x MG
Ligand PO4 4 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 65.963Å b: 98.457Å c: 152.146Å
α: 90° β: 94.61° γ: 90°
R-values:
R R work R free
0.149 0.148 0.169
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Why nature really chose phosphate.
Kamerlin et al. (2013)
3 more

11 mentions without citation

High-resolution analysis of Zn(2+) coordination in the alkaline phosphatase superfamily by EXAFS and x-ray crystallography.
Bobyr et al. (2012)
10 more