› 3tgb

X-ray diffraction
1.35Å resolution

Crystal structure of L130R mutant of Nitrophorin 4 from Rhodnius prolixus complexed with imidazole at pH 7.4

Released:
DOI: 10.2210/pdb3tgb/pdb
PDB entry 3tgb coloured by chain, front view.
PDB entry 3tgb coloured by chain, side view.
PDB entry 3tgb coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Guanidine-ferroheme coordination in the mutant protein nitrophorin 4(L130R).
He C, Fuchs MR, Ogata H, Knipp M
Angew Chem Int Ed Engl 51 4470-3 (2012)
PMID: 22334402

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.34 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand IMD 1 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: C2
Unit cell:
a: 69.719Å b: 43.058Å c: 52.469Å
α: 90° β: 94.43° γ: 90°
R-values:
R R work R free
0.145 0.143 0.186
Expression system: Escherichia coli

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Citations

1 review citation

Novel approaches to screening guanidine derivatives.
Rauf et al. (2014)
6 other articles