› 3tgy

X-ray diffraction
2.35Å resolution

Crystal structure of the complex of Bovine Lactoperoxidase with Ascorbic acid at 2.35 A resolution

Released:
DOI: 10.2210/pdb3tgy/pdb
PDB entry 3tgy coloured by chain, front view.
PDB entry 3tgy coloured by chain, side view.
PDB entry 3tgy coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Structure of bovine lactoperoxidase with a partially linked heme moiety at 1.98Å resolution.
Singh PK, Sirohi HV, Iqbal N, Tiwari P, Kaur P, Sharma S, Singh TP
Biochim Biophys Acta Proteins Proteom 1865 329-335 (2017)
PMID: 27986533

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160329 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lactoperoxidase Chain: A
Molecule details ›
Chain: A
Length: 595 amino acids
Theoretical weight: 67.85 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P80025 (Residues: 118-712; Coverage: 86%)
Gene name: LPO
Sequence domains: Animal haem peroxidase
Structure domains: Haem peroxidase domain superfamily, animal type

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
9 bound ligands:
Ligand CA 1 x CA
Ligand NAG 2 x NAG
Ligand IOD 9 x IOD
Ligand MPD 1 x MPD
Ligand EDO 1 x EDO
Ligand PEG 1 x PEG
Ligand GOL 1 x GOL
Ligand SCN 1 x SCN
Ligand ASC 1 x ASC
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 53.706Å b: 79.614Å c: 65.785Å
α: 90° β: 93.23° γ: 90°
R-values:
R R work R free
0.228 0.226 0.25

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Citations

4 citation in other articles

Pre-steady-state Kinetics Reveal the Substrate Specificity and Mechanism of Halide Oxidation of Truncated Human Peroxidasin 1.
Paumann-Page et al. (2017)
3 more

1 mention without citation

Structural Basis for DNA Recognition of a Single-stranded DNA-binding Protein from Enterobacter Phage Enc34.
Cernooka et al. (2017)