› 3thk

X-ray diffraction
1.7Å resolution

Structure of SH3 chimera with a type II ligand linked to the chain C-terminal

Released:
DOI: 10.2210/pdb3thk/pdb
Model geometry
Fit model/data
PDB entry 3thk coloured by chain, front view.
PDB entry 3thk coloured by chain, side view.
PDB entry 3thk coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
High-resolution crystal structure of spectrin SH3 domain fused with a proline-rich peptide.
Gushchina LV, Gabdulkhakov AG, Nikonov SV, Filimonov VV
J Biomol Struct Dyn 29 485-95 (2011)
PMID: 22066535

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147596 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Spectrin alpha chain, non-erythrocytic 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 73 amino acids
Theoretical weight: 8.21 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16086 (Residues: 967-1035; Coverage: 3%)
Gene names: Spna2, Spta2, Sptan1
Sequence domains: SH3 domain
Structure domains: SH3 Domains
Proline-rich peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 amino acids
Theoretical weight: 981 Da
Source organism: Rattus norvegicus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand BME 2 x BME
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X12
Spacegroup: P32
Unit cell:
a: 36.38Å b: 36.38Å c: 112.14Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.18 0.201
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Spectrin: structure, function and disease.
Zhang et al. (2013)
4 other articles

3 mentions without citation

Co-Evolutionary Fitness Landscapes for Sequence Design.
Tian et al. (2018)
2 more