› 3thr

X-ray diffraction
2Å resolution

Crystal structure of rat native liver Glycine N-methyltransferase complexed with 5-methyltetrahydrofolate monoglutamate

Released:
DOI: 10.2210/pdb3thr/pdb
PDB entry 3thr coloured by chain, front view.
PDB entry 3thr coloured by chain, side view.
PDB entry 3thr coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Differences in folate-protein interactions result in differing inhibition of native rat liver and recombinant glycine N-methyltransferase by 5-methyltetrahydrofolate.
Luka Z, Pakhomova S, Loukachevitch LV, Newcomer ME, Wagner C
Biochim Biophys Acta 1824 286-91 (2012)
PMID: 22037183

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + glycine = S-adenosyl-L-homocysteine + sarcosine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146529 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycine N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 293 amino acids
Theoretical weight: 32.49 KDa
Source organism: Rattus norvegicus
UniProt:
  • Canonical: P13255 (Residues: 2-293; Coverage: 100%)
Gene names: Fbp-cII, Gnmt
Sequence domains: Methyltransferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand C2F 4 x C2F
Ligand TAM 3 x TAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21
Unit cell:
a: 59.186Å b: 83.788Å c: 134.436Å
α: 90° β: 91.32° γ: 90°
R-values:
R R work R free
0.181 0.178 0.241

Quick links

Citations

2 review citations

Sarcosine as a potential prostate cancer biomarker--a review.
Cernei et al. (2013)
1 more

4 mentions without citation

Tertiary structure of Escherichia coli tRNA(3Thr) in solution and interaction of this tRNA with the cognate threonyl-tRNA synthetase.
Theobald et al. (1988)
3 more