› 3thu

X-ray diffraction
1.8Å resolution

Crystal structure of an enolase from sphingomonas sp. ska58 (efi target efi-501683) with bound mg

Released:
DOI: 10.2210/pdb3thu/pdb
PDB entry 3thu coloured by chain, front view.
PDB entry 3thu coloured by chain, side view.
PDB entry 3thu coloured by chain, top view.
Source organism: Sphingomonas sp. SKA58
Entry authors: Vetting MW, Toro R, Bhosle R, Wasserman SR, Morisco LL, Hillerich B, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172978 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-mannonate dehydratase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 426 amino acids
Theoretical weight: 47.71 KDa
Source organism: Sphingomonas sp. SKA58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q1NAJ2 (Residues: 1-403; Coverage: 100%)
Gene name: SKA58_08619
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand CL 2 x CL
Ligand GOL 8 x GOL
Ligand UNX 6 x UNX
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I422
Unit cell:
a: 124.799Å b: 124.799Å c: 343.092Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.14 0.138 0.165
Expression system: Escherichia coli BL21(DE3)

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