› 3ti2

X-ray diffraction
1.9Å resolution

1.90 Angstrom resolution crystal structure of N-terminal domain 3-phosphoshikimate 1-carboxyvinyltransferase from Vibrio cholerae

Released:
DOI: 10.2210/pdb3ti2/pdb
PDB entry 3ti2 coloured by chain, front view.
PDB entry 3ti2 coloured by chain, side view.
PDB entry 3ti2 coloured by chain, top view.
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Entry authors: Light SH, Minasov G, Halavaty AS, Shuvalova L, Papazisi L, Anderson WF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192057 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoshikimate 1-carboxyvinyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 228 amino acids
Theoretical weight: 24.54 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9KRB0 (Residues: 16-243; Coverage: 54%)
Gene names: VC_1732, aroA
Sequence domains: EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Structure domains: Enolpyruvate transferase domain

Ligands and Environments

2 bound ligands:
Ligand CL 11 x CL
Ligand PG4 2 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 77.758Å b: 40.072Å c: 133.861Å
α: 90° β: 90.33° γ: 90°
R-values:
R R work R free
0.153 0.151 0.196
Expression system: Escherichia coli BL21

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