› 3tin

X-ray diffraction
2.9Å resolution

Tubulin tyrosine ligase

Released:
DOI: 10.2210/pdb3tin/pdb
PDB entry 3tin coloured by chain, front view.
PDB entry 3tin coloured by chain, side view.
PDB entry 3tin coloured by chain, top view.
Source organism: Xenopus tropicalis
Primary publication:
Tubulin tyrosine ligase structure reveals adaptation of an ancient fold to bind and modify tubulin.
Szyk A, Deaconescu AM, Piszczek G, Roll-Mecak A
Nat Struct Mol Biol 18 1250-8 (2011)
PMID: 22020298

Function and Biology Details

Reaction catalysed: 
ATP + detyrosinated alpha-tubulin + L-tyrosine = alpha-tubulin + ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123656 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tubulin--tyrosine ligase Chain: A
Molecule details ›
Chain: A
Length: 380 amino acids
Theoretical weight: 43.74 KDa
Source organism: Xenopus tropicalis
Expression system: Escherichia coli
UniProt:
  • Canonical: F6Z895 (Residues: 2-377; Coverage: 100%)
Gene name: ttl
Sequence domains: Tubulin-tyrosine ligase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 116.97Å b: 75.72Å c: 44.23Å
α: 90° β: 90.83° γ: 90°
R-values:
R R work R free
0.232 0.232 0.313
Expression system: Escherichia coli

Quick links

Citations

24 review citations

The tubulin code: molecular components, readout mechanisms, and functions.
Janke C. (2014)
23 more