› 3tjy

X-ray diffraction
1.7Å resolution

Structure of the Pto-binding domain of HopPmaL generated by limited chymotrypsin digestion

Released:
DOI: 10.2210/pdb3tjy/pdb
PDB entry 3tjy coloured by chain, front view.
PDB entry 3tjy coloured by chain, side view.
PDB entry 3tjy coloured by chain, top view.
Source organism: Pseudomonas syringae pv. maculicola str. ES4326
Primary publication:
Structural analysis of HopPmaL reveals the presence of a second adaptor domain common to the HopAB family of Pseudomonas syringae type III effectors.
Singer AU, Wu B, Yee A, Houliston S, Xu X, Cui H, Skarina T, Garcia M, Semesi A, Arrowsmith CH, Savchenko A
Biochemistry 51 1-3 (2012)
PMID: 22191472

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186015 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Effector protein hopAB3 Chain: A
Molecule details ›
Chain: A
Length: 94 amino acids
Theoretical weight: 10.66 KDa
Source organism: Pseudomonas syringae pv. maculicola str. ES4326
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RP04 (Residues: 140-233; Coverage: 24%)
Gene names: hopAB3, hopPmaL
Structure domains: Monooxygenase

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand CL 4 x CL
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 57.427Å b: 57.427Å c: 54.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.188 0.217
Expression system: Escherichia coli

Quick links

Citations

2 review citations

On the front line: structural insights into plant-pathogen interactions.
Wirthmueller et al. (2013)
1 more

2 mentions without citation

The geometry of Niggli reduction: SAUC - search of alternative unit cells.
McGill et al. (2014)
1 more