› 3tk1

X-ray diffraction
2.4Å resolution

Crystal structure of a MeaB and Rv1496 ortholog from Mycobacterium thermoresistible bound to GDP

Released:
DOI: 10.2210/pdb3tk1/pdb
Model geometry
Fit model/data
PDB entry 3tk1 coloured by chain, front view.
PDB entry 3tk1 coloured by chain, side view.
PDB entry 3tk1 coloured by chain, top view.
Source organism: Mycolicibacterium thermoresistibile ATCC 19527
Primary publication:
Crystal structures of Mycobacterial MeaB and MMAA-like GTPases.
Edwards TE, Baugh L, Bullen J, Baydo RO, Witte P, Thompkins K, Phan IQ, Abendroth J, Clifton MC, Sankaran B, Van Voorhis WC, Myler PJ, Staker BL, Grundner C, Lorimer DD
J Struct Funct Genomics 16 91-9 (2015)
PMID: 25832174

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124539 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Membrane ATPase/protein kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 35.67 KDa
Source organism: Mycolicibacterium thermoresistibile ATCC 19527
Expression system: Escherichia coli
UniProt:
  • Canonical: G7CAR0 (Residues: 1-326; Coverage: 100%)
Gene name: KEK_00260
Sequence domains: Methylmalonyl Co-A mutase-associated GTPase MeaB
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand GDP 2 x GDP
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P212121
Unit cell:
a: 42.38Å b: 106.43Å c: 134.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.224 0.277
Expression system: Escherichia coli

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Citations

2 review citations

Metallochaperones and metalloregulation in bacteria.
Capdevila et al. (2017)
1 more

3 mentions without citation

Mycobacterium thermoresistibile as a source of thermostable orthologs of Mycobacterium tuberculosis proteins.
Edwards et al. (2012)
2 more