› 3tky

X-ray diffraction
2.47Å resolution

Monolignol o-methyltransferase (momt)

Released:
DOI: 10.2210/pdb3tky/pdb
PDB entry 3tky coloured by chain, front view.
PDB entry 3tky coloured by chain, side view.
PDB entry 3tky coloured by chain, top view.
Source organism: Clarkia breweri
Primary publication:
An engineered monolignol 4-o-methyltransferase depresses lignin biosynthesis and confers novel metabolic capability in Arabidopsis.
Zhang K, Bhuiya MW, Pazo JR, Miao Y, Kim H, Ralph J, Liu CJ
Plant Cell 24 3135-52 (2012)
PMID: 22851762

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + isoeugenol = S-adenosyl-L-homocysteine + isomethyleugenol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126445 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(Iso)eugenol O-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 368 amino acids
Theoretical weight: 39.99 KDa
Source organism: Clarkia breweri
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O04385 (Residues: 1-368; Coverage: 100%)
Gene name: IEMT1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
1 bound ligand:
Ligand N7I 4 x N7I
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 65.855Å b: 152.16Å c: 68.181Å
α: 90° β: 94.92° γ: 90°
R-values:
R R work R free
0.197 0.193 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Lignin valorization: improving lignin processing in the biorefinery.
Ragauskas et al. (2014)
15 more

2 mentions without citation

Functional Diversification and Structural Origins of Plant Natural Product Methyltransferases.
Lashley et al. (2022)
1 more