› 3tl2

X-ray diffraction
1.7Å resolution

Crystal structure of Bacillus anthracis str. Ames malate dehydrogenase in closed conformation.

Released:
DOI: 10.2210/pdb3tl2/pdb
PDB entry 3tl2 coloured by chain, front view.
PDB entry 3tl2 coloured by chain, side view.
PDB entry 3tl2 coloured by chain, top view.
Source organism: Bacillus anthracis
Entry authors: Blus BJ, Chruszcz M, Tkaczuk KL, Osinski T, Cymborowski M, Kudritska M, Grimshaw S, Savchenko A, Anderson WF, Minor W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
(S)-malate + NAD(+) = oxaloacetate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179744 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Malate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 33.81 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6HSF4 (Residues: 1-312; Coverage: 100%)
Gene names: BAS4486, BA_4837, GBAA_4837, mdh
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 2 x EDO
Ligand SCN 2 x SCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: I222
Unit cell:
a: 67.181Å b: 68.377Å c: 142.919Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.198
Expression system: Escherichia coli BL21(DE3)

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