› 3tlj

X-ray diffraction
2.2Å resolution

Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosyl-L-homocysteine

Released:
DOI: 10.2210/pdb3tlj/pdb
PDB entry 3tlj coloured by chain, front view.
PDB entry 3tlj coloured by chain, side view.
PDB entry 3tlj coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.
Fislage M, Roovers M, Tuszynska I, Bujnicki JM, Droogmans L, Versées W
Nucleic Acids Res 40 5149-61 (2012)
PMID: 22362751

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(6) in tRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(6) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186433 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine(6)-N2)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 373 amino acids
Theoretical weight: 42.13 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U248 (Residues: 1-365; Coverage: 100%)
Gene names: PF1002, trm14
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SAH 2 x SAH
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21
Unit cell:
a: 82.52Å b: 45.33Å c: 122.23Å
α: 90° β: 91.23° γ: 90°
R-values:
R R work R free
0.187 0.186 0.22
Expression system: Escherichia coli

Quick links

Citations

8 review citations

How RNA-Binding Proteins Interact with RNA: Molecules and Mechanisms.
Corley et al. (2020)
7 more

2 mentions without citation

Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life.
Fislage et al. (2012)
1 more