› 3tlm

X-ray diffraction
2.95Å resolution

Crystal Structure of Endoplasmic Reticulum Ca2+-ATPase (SERCA) From Bovine Muscle

Released:
DOI: 10.2210/pdb3tlm/pdb
PDB entry 3tlm coloured by chain, front view.
PDB entry 3tlm coloured by chain, side view.
PDB entry 3tlm coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structure of sarcoplasmic reticulum Ca2+-ATPase (SERCA) from bovine muscle.
Sacchetto R, Bertipaglia I, Giannetti S, Cendron L, Mascarello F, Damiani E, Carafoli E, Zanotti G
J Struct Biol 178 38-44 (2012)
PMID: 22387132

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + Ca(2+)(Side 1) = ADP + phosphate + Ca(2+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171172 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 Chain: A
Molecule details ›
Chain: A
Length: 992 amino acids
Theoretical weight: 109.35 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q0VCY0 (Residues: 1-992; Coverage: 100%)
Gene name: ATP2A1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand CA 2 x CA
Ligand MG 1 x MG
Ligand K 1 x K
Ligand ACP 1 x ACP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 156.58Å b: 75.26Å c: 151.8Å
α: 90° β: 108.3° γ: 90°
R-values:
R R work R free
0.205 0.205 0.277

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Citations

5 review citations

Regulation of Calcium Homeostasis by ER Redox: A Close-Up of the ER/Mitochondria Connection.
Chernorudskiy et al. (2017)
4 more

10 mentions without citation

Linking Biochemical and Structural States of SERCA: Achievements, Challenges, and New Opportunities.
Aguayo-Ortiz et al. (2020)
9 more