› 3tls

X-ray diffraction
3.2Å resolution

The GLIC pentameric Ligand-Gated Ion Channel E19'P mutant in a locally-closed conformation (LC2 subtype)

Released:
DOI: 10.2210/pdb3tls/pdb
PDB entry 3tls coloured by chain, front view.
PDB entry 3tls coloured by chain, side view.
PDB entry 3tls coloured by chain, top view.
Source organism: Gloeobacter violaceus
Primary publication:
A locally closed conformation of a bacterial pentameric proton-gated ion channel.
Prevost MS, Sauguet L, Nury H, Van Renterghem C, Huon C, Poitevin F, Baaden M, Delarue M, Corringer PJ
Nat Struct Mol Biol 19 642-9 (2012)
PMID: 22580559

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181806 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 321 amino acids
Theoretical weight: 36.55 KDa
Source organism: Gloeobacter violaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 44-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 5 x CL
Ligand LMT 1 x LMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: C2
Unit cell:
a: 180.272Å b: 131.074Å c: 158.139Å
α: 90° β: 101.92° γ: 90°
R-values:
R R work R free
0.205 0.203 0.23
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
19 more

8 mentions without citation

Structure and Pharmacologic Modulation of Inhibitory Glycine Receptors.
Burgos et al. (2016)
7 more